Journal: Journal of Animal Science and Biotechnology

Article Title: RNA-seq analysis reveals the critical role of the novel lncRNA BIANCR in intramuscular adipogenesis through the ERK1/2 signaling pathway

doi: 10.1186/s40104-022-00820-1

Figure Lengend Snippet: BIANCR promoted the proliferation and inhibited the apoptosis of intramuscular preadipocytes A EdU staining measured the number of intramuscular preadipocytes at proliferative stages. B Cell vitality was evaluated by CCK-8 assay. C The number of cells in G1(Gap 1 phase), S (Synthesis phase), and G2 (Gap 2 phase) were calculated by flow cytometry. D The mRNA expression levels of cell cycle related genes ( CDK1 , CDK2 , CCND2 , PCNA , p21 , and p27 ). E The protein expression levels of cell cycle related genes (CDK1, PCNA, p21, and p27). F The number of viable non-apoptotic cells, viable apoptotic cells, non-viable apoptotic cells, and non-viable cells were counted by flow cytometry. G The mRNA expression levels of apoptotic related genes ( BAD , CASP3 , CASP6 , BCL2 and BCL2L1 ). ( H ) The protein expression levels of apoptotic related genes (BAD, CASP3, and BCL2L1). Results are presented as the means ± SD, * P < 0.05; ** P < 0.01

Article Snippet: The intramuscular preadipocytes were treated according to the protocol of the Cell Cycle Staining Kit (Multisciences, Hangzhou, China) and the Annexin V-FITC/PI Apoptosis Kit (Multisciences, Hangzhou, China) 48 h after transfection.

Techniques: Staining, CCK-8 Assay, Flow Cytometry, Expressing

Journal: Experimental and Therapeutic Medicine

Article Title: Scutellaria barbata D. Don inhibits growth and induces apoptosis by suppressing IL-6-inducible STAT3 pathway activation in human colorectal cancer cells

doi: 10.3892/etm.2015.2692

Figure Lengend Snippet: Effect of EESB on the HT-29 cell cycle. Cells were pretreated with various concentration of EESB for 1 h, followed by stimulation with 10 ng/ml IL-6 for 24 h. (A) Cells were stained with propidium iodide and analyzed by fluorescence-activated cell sorting. The proportion of DNA in the S phase was calculated using ModFit LT version 3.0 software. (B) Quantification of fluorescence-activated cell sorting analysis. The data shown are averages with standard deviation from 3 independent experiments. #P<0.05 vs. cells treated with IL-6 but not EESB. EESB, ethanol extract of Scutellaria barbata D. Don; IL-6, interleukin-6.

Article Snippet: The HT-29 cell cycle progression was determined through flow cytometric analysis using a propidium iodide (PI) staining cell cycle assay kit (BD Biosciences, Franklin Lakes, NJ, USA).

Techniques: Concentration Assay, Staining, Fluorescence, FACS, Software, Standard Deviation

Journal: Experimental and Therapeutic Medicine

Article Title: Scutellaria barbata D. Don inhibits growth and induces apoptosis by suppressing IL-6-inducible STAT3 pathway activation in human colorectal cancer cells

doi: 10.3892/etm.2015.2692

Figure Lengend Snippet: Effect of EESB on HT-29 cell apoptosis. Cells were pretreated with various concentration of EESB for 1 h, followed by stimulation with 10 ng/ml IL-6 for 24 h. (A) Cells were collected and stained with Annexin V/PI, followed by fluorescence-activated cell sorting analysis. Double-negative stained cells indicate the live cell population; Annexin V-positive/PI-negative stained cells and Annexin V/PI double-positive stained cells represent early and late apoptosis, respectively; Annexin V-negative and PI-positive stained cells show dead cells. (B) Quantification of fluorescence-activated cell sorting analysis. The data shown are averages with standard deviation from 3 independent experiments. #P<0.05 vs. cells treated with IL-6 but not EESB. EESB, ethanol extract of Scutellaria barbata D. Don; IL-6, interleukin-6; UL, upper left; UR, upper right; LR, lower right; LL, lower left; PI, propidium iodide; FITC, fluorescein isothiocyanate.

Article Snippet: The HT-29 cell cycle progression was determined through flow cytometric analysis using a propidium iodide (PI) staining cell cycle assay kit (BD Biosciences, Franklin Lakes, NJ, USA).

Techniques: Concentration Assay, Staining, Fluorescence, FACS, Standard Deviation